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Product
Parameter
B-complex vitamins
SOP No.
LAB_P_SOP_272
Date of Analysis
01.01.2015
Equipment - Sample Preparation
Electronic Balance
BLR/PS-CLF/007.02
Vortex
Homogenizer
Refrigerated Centrifuge
BLR/PS-CLF/005.02
pH meter
BLR/FCL/010.02
Equipment - Screening/Quantification
LC-MS/MS-Agilent -6460C
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Chemicals
Name- Grade & Brand
Ascorbic acid- AR grade- Merck
Milli-Q water
Ammonia, AR grade - Merck
Formic acid, MS grade
Ammonium Formate, MS grade
Reference Material -Preparation
Sample Preparation
Weight of Sample
0.25 g
Volume made
50.0 ml
Aliquot Taken
1.0 ml
Final Dilution
1.0 ml
Validation Plan Criteria
Refer Page No. 3 and 4
Conclusion
Performed by
Manjunath K
Reviewed by
BKVR Prasad
Sign & Date
Approved by
M.Meena
GM-Chemical Testing
Sign & Date
Page 2
Validation Report No. : BLR/PS-FRL/VAL/XXX
Validation is performed as per AOAC - SPIFAN guidelines to verify the method for its performance. Method is found to be suitable to perform the B-complex vitamins in
Infant Formulas and Dietary Supplements.
Infant Formula, Dietary Supplements, Fruit juices
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE