154 / 670
Validation Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Equiment
Analyst
Sample Blank No.
Retention Time (min)
m/z
Standard (Peak Area)
Blank (Peak Area)
Area % of blank to
lowest calibrant
(Criteria = <30%)
Lowest calibrant
concentration ug/l
1
1.867
124.2>80.1
3069.0
653.0
21.28
5.0
2
1.867
124.2>80.1
3032.7
653.0
21.53
5.0
3
1.867
124.2>80.1
2779.1
653.0
23.50
5.0
4
1.867
124.2>80.1
3012.4
653.0
21.68
5.0
5
1.867
124.2>80.1
3343.1
653.0
19.53
5.0
6
1.867
124.2>80.1
3230.8
653.0
20.21
5.0
7
1.867
124.2>80.1
3161.2
653.0
20.66
5.0
8
1.867
124.2>80.1
2919.9
653.0
22.36
5.0
9
1.867
124.2>80.1
2933.1
653.0
22.26
5.0
10
1.867
124.2>80.1
3066.1
653.0
21.30
5.0
Mean
1.87
Mean
21.431
Concentration Analyte Retention
Time (Minutes)
124.2 > 78.1
Ion Ratio
% Relative
Intensity
RT Difference
(criteria = ± 0.2
minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
1.867
3013.099027
2907
0.965
96.5
Pass
Confirmed
Calibration standard 2
1.867
5670.810404
5539
0.977
97.7
Pass
Confirmed
Calibration standard 3
1.867
26674.59717
24536
0.920
92.0
Pass
Confirmed
Calibration standard 4
1.867
50125.53391
45199
0.902
90.2
Pass
Confirmed
Calibration standard 5
1.867
92926.1074
85044
0.915
91.5
Pass
Confirmed
Calibration standard 6
1.867
186487.7079
162873
0.873
87.3
Pass
Confirmed
Mean
1.867
Eezee paste-1
1.867
21884.60
20694.00
0.95
95
0.000
Pass
Confirmed
Eezee paste-2
1.867
21997.95
20833.00
0.95
95
0.000
Pass
Confirmed
Eezee paste-3
1.867
22494.65
21173.00
0.94
94
0.000
Pass
Confirmed
Eezee paste-4
1.867
22371.28
20886.00
0.93
93
0.000
Pass
Confirmed
Eezee paste-5
1.867
22131.94
20807.00
0.94
94
0.000
Pass
Confirmed
Eezee paste-6
1.867
21606.36
20389.00
0.94
94
0.000
Pass
Confirmed
Average ion ratio
0.93
Relative intensity (%
of base peak)
% Tolerance plus or
minus
Average intensity
93
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.65
>10-20
30
Upper limit
1.20
< 10
30
Page 8
AOAC- SPIFAN-2012-VITAMINS
Method Blank
TUV/BLR/F-360
LC-MS/MS
Manjunath K/Prasad BKVR
Specificity
B-Complex Vitamins
01.01.2015
Nicotinic acid
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE