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Validation Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Equiment
Analyst
Sample Blank No.
Retention Time (min)
m/z
Standard (Peak Area)
Blank (Peak Area)
Area % of blank to
lowest calibrant
(Criteria = <30%)
Lowest calibrant
concentration ug/l
1
2.843
220.1>90.0
9257.6
2682.0
28.97
5.0
2
2.843
220.1>90.0
8551.1
2682.0
31.36
5.0
3
2.843
220.1>90.0
8818.9
2682.0
30.41
5.0
4
2.843
220.1>90.0
8773.9
2682.0
30.57
5.0
5
2.843
220.1>90.0
9107.2
2682.0
29.45
5.0
6
2.843
220.1>90.0
9144.9
2682.0
29.33
5.0
7
2.843
220.1>90.0
9323.9
2682.0
28.76
5.0
8
2.843
220.1>90.0
8828.1
2682.0
30.38
5.0
9
2.843
220.1>90.0
8738.3
2682.0
30.69
5.0
10
2.843
220.1>90.0
8949.5
2682.0
29.97
5.0
Mean
2.84
Mean
29.990
Concentration Analyte Retention
Time (Minutes)
220.1>202.1
Ion Ratio
% Relative
Intensity
RT Difference
(criteria = ± 0.2
minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
2.843
9491.885498
3459
0.364
36.4
Pass
Confirmed
Calibration standard 2
2.843
18222.57862
6438
0.353
35.3
Pass
Confirmed
Calibration standard 3
2.843
93229.19028
33162
0.356
35.6
Pass
Confirmed
Calibration standard 4
2.843
181246.3689
65844
0.363
36.3
Pass
Confirmed
Calibration standard 5
2.843
355269.3599
130367
0.367
36.7
Pass
Confirmed
Calibration standard 6
2.843
700583.3038
251451
0.359
35.9
Pass
Confirmed
Mean
2.843
SRM 1849a-1
2.843
587092.12
212140.00
0.36
36
0.000
Pass
Confirmed
SRM 1849a-2
2.843
596359.89
215759.00
0.36
36
0.000
Pass
Confirmed
SRM 1849a-3
2.843
591637.57
209899.00
0.35
35
0.000
Pass
Confirmed
SRM 1849a-4
2.843
605049.95
217663.00
0.36
36
0.000
Pass
Confirmed
SRM 1849a-5
2.843
592662.67
214701.00
0.36
36
0.000
Pass
Confirmed
SRM 1849a-6
2.843
588123.52
211619.00
0.36
36
0.000
Pass
Confirmed
Average ion ratio
0.36
Relative intensity (% of
base peak)
% Tolerance plus or
minus
Average intensity
36
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.25
>10-20
30
Upper limit
0.47
< 10
30
Page 9
Method Blank
TUV/BLR/F-360
LC-MS/MS
Manjunath K/Prasad BKVR
AOAC- SPIFAN-2012-VITAMINS
Specificity
B-Complex Vitamins
01.01.2015
Pantothenic acid
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE