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Validation Parameter

Test Group Name

Date

Test Name

Data Path File

Matrix

Raw-Data File Ref.

Equiment

Analyst

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

Area % of blank to

lowest calibrant

(Criteria = <30%)

Lowest calibrant

concentration ug/l

1

3.333

442.2>295

1323.4

187.0

14.13

2.0

2

3.333

442.2>295

1359.8

187.0

13.75

2.0

3

3.333

442.2>295

1363.8

187.0

13.71

2.0

4

3.333

442.2>295

1268.4

187.0

14.74

2.0

5

3.333

442.2>295

1310.4

187.0

14.27

2.0

6

3.333

442.2>295

1276.8

187.0

14.65

2.0

7

3.333

442.2>295

1325.8

187.0

14.10

2.0

8

3.333

442.2>295

1342.0

187.0

13.93

2.0

9

3.333

442.2>295

1518.9

187.0

12.31

2.0

10

3.333

442.2>295

1359.0

187.0

13.76

2.0

Mean

3.33

Mean

13.937

Concentration Analyte Retention

Time (Minutes)

442.2>176.0

Ion Ratio

% Relative

Intensity

RT Difference

(criteria = ± 0.2

minute)

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail

Calibration standard 1

3.333

2975.057627

907

0.305

30.5

Pass

Confirmed

Calibration standard 2

3.333

5939.641659

1883

0.317

31.7

Pass

Confirmed

Calibration standard 3

3.333

30455.02702

9337

0.307

30.7

Pass

Confirmed

Calibration standard 4

3.333

63141.05518

19441

0.308

30.8

Pass

Confirmed

Calibration standard 5

3.333

135368.6573

41994

0.310

31.0

Pass

Confirmed

Calibration standard 6

3.333

365563.4524

112807

0.309

30.9

Pass

Confirmed

Mean

3.333

SRM 1849a-1

3.333

7844.17

2564.00

0.327

32.7

0.000

Pass

Confirmed

SRM 1849a-2

3.333

8277.19

2512.00

0.303

30.3

0.000

Pass

Confirmed

SRM 1849a-3

3.333

7934.49

2570.00

0.324

32.4

0.000

Pass

Confirmed

SRM 1849a-4

3.333

7983.66

2385.00

0.299

29.9

0.000

Pass

Confirmed

SRM 1849a-5

3.333

8153.55

2435.00

0.299

29.9

0.000

Pass

Confirmed

SRM 1849a-6

3.333

7529.87

2559.00

0.340

34.0

0.000

Pass

Confirmed

Average ion ratio

0.31

Relative intensity (%

of base peak)

% Tolerance plus or

minus

Average intensity

31

>50

30

Tolerance (%)

30.00

>20-50

30

Lower limit

0.22

>10-20

30

Upper limit

0.40

< 10

30

Page 12

Method Blank

TUV/BLR/F-360

LC-MS/MS

Manjunath K/Prasad BKVR

AOAC- SPIFAN-2012-VITAMINS

Specificity

B-Complex Vitamins

01.01.2015

Folic acid

2015\January\Vitamins\01.01.2015

BVit-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE