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Validation Parameter

Test Group Name

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

With Multilevel Calibration

Calibration Standards

Concentration (ug/kg)

Area-Analyte

Solvent Standard Level-1

5.00

13753.35

Solvent Standard Level-2

10.00

25632.40

Solvent Standard Level-3

50.00

114620.22

Solvent Standard Level-4

100.00

216982.92

Solvent Standard Level-5

300.00

583442.21

Solvent Standard Level-6

500.00

902435.56

Criteria

R

2

≥ 0.99

Conclusion

Complies

Page no. 15

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

B-complex Vitamins

Niacinamide

Aqueous

y = 1800x + 19977

R² = 0.9976

0.00

100000.00

200000.00

300000.00

400000.00

500000.00

600000.00

700000.00

800000.00

900000.00

1000000.00

0.00

100.00 200.00 300.00 400.00 500.00 600.00

BVit-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE