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Validation Paramete

Test Group Name

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Re

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

With Multilevel Calibration

Calibration Standards

Concentration

(ug/kg)

Area-Analyte

Solvent Standard Level-1

2.00

1113.31

Solvent Standard Level-2

5.00

2659.72

Solvent Standard Level-3

20.00

10952.16

Solvent Standard Level-4

50.00

27674.14

Solvent Standard Level-5

100.00

59896.08

Solvent Standard Level-6

200.00

127588.73

Criteria

R

2

≥ 0.99

Conclusion

Complies

Page 20

LC-MS/MS 6460A (BLR/PS-CRS/006.03

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

B-complex Vitamins

Folic acid

Aqueous

y = 638.88x - 1828.6

R² = 0.9985

-20000.00

0.00

20000.00

40000.00

60000.00

80000.00

100000.00

120000.00

140000.00

0.00

50.00 100.00 150.00 200.00 250.00

BVit-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE