167 / 670
Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 5 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
1.823
265.2>122.2
4.608
Mean (μg/l)
4.44
2
1.823
265.2>122.2
4.785
SD
0.3339
3
1.823
265.2>122.2
4.453
LOD (Mean +3 sigma)
5.45
4
1.823
265.2>122.2
4.814
LOQ (Mean + 10sigma)
7.78
5
1.823
265.2>122.2
4.194
RSD %
7.51
6
1.823
265.2>122.2
4.135
Standard Conc. (μg/l)
5
7
1.823
265.2>122.2
4.675
Accuracy %
88.9
8
1.823
265.2>122.2
4.774
9
1.823
265.2>122.2
4.099
Accuracy = 70 - 125 %
10
1.823
265.2>122.2
3.911
RSD% = <15%
Page 21
Criteria
Caluclation
TUV/BLR/F-360
2015\January\Vitamins\01.01.2015
01.01.2015
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
B-Complex vitamins
Thiamine
Aqueous
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Prasad BKVR/Kesav Kumar N
Manjunath K/Prasad BKVR
TUV/BLR/F-99
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE