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Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 5 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
2.315
123.2>80.3
4.949
Mean (μg/l)
4.73
2
2.315
123.2>80.3
4.916
SD
0.1886
3
2.315
123.2>80.3
4.539
LOD (Mean +3 sigma)
5.29
4
2.315
123.2>80.3
4.639
LOQ (Mean + 10sigma)
6.61
5
2.315
123.2>80.3
4.896
RSD %
3.99
6
2.315
123.2>80.3
4.580
Standard Conc. (μg/l)
5
7
2.315
123.2>80.3
4.966
Accuracy %
94.5
8
2.315
123.2>80.3
4.491
9
2.315
123.2>80.3
4.725
Accuracy = 70 - 125 %
10
2.315
123.2>80.3
4.555
RSD% = <15%
Page 23
Criteria
Caluclation
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
B-Complex vitamins
Niacinamide
Aqueous
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
01.01.2015
2015\January\Vitamins\01.01.2015
Prasad BKVR/Kesav Kumar N
TUV/BLR/F-360
TUV/BLR/F-99
Manjunath K/Prasad BKVR
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE