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Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 2 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
3.333
442.2>295
1.970
Mean (μg/l)
2.01
2
3.333
442.2>295
2.034
SD
0.1206
3
3.333
442.2>295
2.040
LOD (Mean +3 sigma)
2.37
4
3.333
442.2>295
1.875
LOQ (Mean + 10sigma)
3.21
5
3.333
442.2>295
1.948
RSD %
6.01
6
3.333
442.2>295
1.890
Standard Conc. (μg/l)
2
7
3.333
442.2>295
1.975
Accuracy %
100.4
8
3.333
442.2>295
2.003
9
3.333
442.2>295
2.309
Accuracy = 70 - 125 %
10
3.333
442.2>295
2.032
RSD% = <15%
Page 28
Caluclation
Criteria
Prasad BKVR/Kesav Kumar N
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
B-Complex vitamins
Folic acid
2015\January\Vitamins\01.01.2015
01.01.2015
Aqueous
TUV/BLR/F-360
TUV/BLR/F-99
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Manjunath K/Prasad BKVR
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE