173 / 670
Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 2 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
3.803
245.2>227.3
1.874
Mean (μg/l)
1.90
2
3.803
245.2>227.3
1.933
SD
0.0497
3
3.803
245.2>227.3
1.950
LOD (Mean +3 sigma)
2.05
4
3.803
245.2>227.3
1.889
LOQ (Mean + 10sigma)
2.39
5
3.803
245.2>227.3
1.933
RSD %
2.62
6
3.803
245.2>227.3
1.782
Standard Conc. (μg/l)
2
7
3.803
245.2>227.3
1.897
Accuracy %
94.8
8
3.803
245.2>227.3
1.857
9
3.803
245.2>227.3
1.914
Accuracy = 70 - 125 %
10
3.803
245.2>227.3
1.932
RSD% = <15%
Page 27
Criteria
Caluclation
Prasad BKVR/Kesav Kumar N
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
B-Complex vitamins
Biotin
2015\January\Vitamins\01.01.2015
01.01.2015
Aqueous
TUV/BLR/F-360
TUV/BLR/F-99
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Manjunath K/Prasad BKVR
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE