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Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 5 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
2.843
220.1>90.0
4.437
Mean (μg/l)
4.23
2
2.843
220.1>90.0
3.960
SD
0.1689
3
2.843
220.1>90.0
4.141
LOD (Mean +3 sigma)
4.74
4
2.843
220.1>90.0
4.110
LOQ (Mean + 10sigma)
5.92
5
2.843
220.1>90.0
4.335
RSD %
3.99
6
2.843
220.1>90.0
4.361
Standard Conc. (μg/l)
5
7
2.843
220.1>90.0
4.482
Accuracy %
84.6
8
2.843
220.1>90.0
4.147
9
2.843
220.1>90.0
4.086
Accuracy = 70 - 125 %
10
2.843
220.1>90.0
4.229
RSD% = <15%
Page 25
Criteria
Caluclation
Prasad BKVR/Kesav Kumar N
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
B-Complex vitamins
Pantothenic acid
2015\January\Vitamins\01.01.2015
01.01.2015
Aqueous
TUV/BLR/F-360
TUV/BLR/F-99
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Manjunath K/Prasad BKVR
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE