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Validation Parameter

Test Group Name

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Ref.

TUV/BLR/F-360

Equiment

Analyst

Manjunath K/Prasad BKVR

With Multilevel Calibration

Calibration Standards

Concentration (ug/kg)

Area-Analyte

Solvent Standard Level-1

5.00

2477.66

Solvent Standard Level-2

10.00

4656.99

Solvent Standard Level-3

50.00

22902.51

Solvent Standard Level-4

100.00

41547.43

Solvent Standard Level-5

200.00

77155.21

Solvent Standard Level-6

300.00

113644.70

Criteria

R

2

≥ 0.99

Conclusion

Complies

Page 16

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

B-complex Vitamins

Nicotinic acid

Aqueous

y = 374.38x + 2237.4

R² = 0.9987

0.00

20000.00

40000.00

60000.00

80000.00

100000.00

120000.00

140000.00

0.00

50.00 100.00 150.00 200.00 250.00 300.00 350.00

BVit-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE