157 / 670
Validation Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Equiment
Analyst
Sample Blank No.
Retention Time (min)
m/z
Standard (Peak Area)
Blank (Peak Area)
Area % of blank to
lowest calibrant
(Criteria = <30%)
Lowest calibrant
concentration ug/l
1
3.838
245.2>227.3
18810.3
5425.0
28.84
2.0
2
3.838
245.2>227.3
18322.9
5659.0
30.88
2.0
3
3.856
245.2>227.3
18722.6
5565.0
29.72
2.0
4
3.856
245.2>227.3
18757.1
5539.0
29.53
2.0
5
3.838
245.2>227.3
18524.8
5586.0
30.15
2.0
6
3.856
245.2>227.3
18848.9
5432.0
28.82
2.0
7
3.856
245.2>227.3
18339.0
5407.0
29.48
2.0
8
3.856
245.2>227.3
18615.1
5728.0
30.77
2.0
9
3.856
245.2>227.3
18471.9
5571.0
30.16
2.0
10
3.856
245.2>227.3
18492.6
5458.0
29.51
2.0
Mean
3.85
Mean
29.788
Concentration Analyte Retention
Time (Minutes)
245.2>97.1
Ion Ratio
% Relative Intensity
RT Difference
(criteria = ± 0.2 minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
3.803
21217.74585
4518
0.213
21.3
Pass
Confirmed
Calibration standard 2
3.803
37598.44009
7591
0.202
20.2
Pass
Confirmed
Calibration standard 3
3.803
167952.8837
32877
0.196
19.6
Pass
Confirmed
Calibration standard 4
3.803
317423.9198
63463
0.200
20.0
Pass
Confirmed
Calibration standard 5
3.803
604434.3372
119692
0.198
19.8
Pass
Confirmed
Calibration standard 6
3.803
1175276.621
233339
0.199
19.9
Pass
Confirmed
Mean
3.803
SRM 1849a-1
3.803
39001.20
7913.00
0.203
20.3
0.000
Pass
Confirmed
SRM 1849a-2
3.803
40053.53
8012.00
0.200
20.0
0.000
Pass
Confirmed
SRM 1849a-3
3.803
39267.12
7777.00
0.198
19.8
0.000
Pass
Confirmed
SRM 1849a-4
3.803
39344.38
7997.00
0.203
20.3
0.000
Pass
Confirmed
SRM 1849a-5
3.803
39895.70
7931.00
0.199
19.9
0.000
Pass
Confirmed
SRM 1849a-6
3.803
39580.06
7830.00
0.198
19.8
0.000
Pass
Confirmed
Average ion ratio
0.20
Relative intensity (%
of base peak)
% Tolerance plus or
minus
Average intensity
20
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.14
>10-20
30
Upper limit
0.26
< 10
30
Page 11
Method Blank
TUV/BLR/F-360
LC-MS/MS
Manjunath K/Prasad BKVR
AOAC- SPIFAN-2012-VITAMINS
Specificity
B-Complex Vitamins
01.01.2015
Biotin
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE