153 / 670
Validation Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Equiment
Analyst
Sample Blank No.
Retention Time (min)
m/z
Standard (Peak Area) Blank (Peak
Area)
Area % of blank to
lowest calibrant
(Criteria = <30%)
Lowest calibrant
concentration ug/l
1
2.315
123.2>80.3
14603.1
3744.0
25.64
5.0
2
2.315
123.2>80.3
14530.7
3744.0
25.77
5.0
3
2.315
123.2>80.3
13703.9
3744.0
27.32
5.0
4
2.315
123.2>80.3
13923.9
3744.0
26.89
5.0
5
2.315
123.2>80.3
14485.7
3744.0
25.85
5.0
6
2.315
123.2>80.3
13793.1
3744.0
27.14
5.0
7
2.315
123.2>80.3
14639.9
3744.0
25.57
5.0
8
2.315
123.2>80.3
13597.7
3744.0
27.53
5.0
9
2.315
123.2>80.3
14111.2
3744.0
26.53
5.0
10
2.315
123.2>80.3
13738.9
3744.0
27.25
5.0
Mean
2.32
Mean
26.550
Concentration Analyte Retention
Time (Minutes)
123.2>53.3
Ion Ratio % Relative
Intensity
RT Difference
(criteria = ± 0.2
minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
2.315
14568.43882
6188
0.425
42.5
Pass
Confirmed
Calibration standard 2
2.315
28956.77542
11076
0.383
38.3
Pass
Confirmed
Calibration standard 3
2.315
114474.3637
50365
0.440
44.0
Pass
Confirmed
Calibration standard 4
2.315
209845.4323
87686
0.418
41.8
Pass
Confirmed
Calibration standard 5
2.315
581016.6522
166838
0.287
28.7
Pass
Confirmed
Calibration standard 6
2.315
875832.1504
368607
0.421
42.1
Pass
Confirmed
Mean
2.315
SRM 1849a-1
2.315
884894.43
328272.00
0.37
37
0.000
Pass
Confirmed
SRM 1849a-2
2.315
899294.52
330416.00
0.37
37
0.000
Pass
Confirmed
SRM 1849a-3
2.315
893797.19
329408.00
0.37
37
0.000
Pass
Confirmed
SRM 1849a-4
2.315
909753.39
330791.00
0.36
36
0.000
Pass
Confirmed
SRM 1849a-5
2.315
892082.67
323780.00
0.36
36
0.000
Pass
Confirmed
SRM 1849a-6
2.315
919462.53
332782.00
0.36
36
0.000
Pass
Confirmed
Average ion ratio
0.40
Relative intensity (%
of base peak)
% Tolerance
plus or minus
Average intensity
40
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.28
>10-20
30
Upper limit
0.51
< 10
30
Page 7
AOAC- SPIFAN-2012-VITAMINS
Method Blank
TUV/BLR/F-360
LC-MS/MS
Manjunath K/Prasad BKVR
Specificity
B-Complex Vitamins
01.01.2015
Niacinamide
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE