151 / 670
Validation Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Equiment
Analyst
Sample Blank No.
Retention Time (min)
m/z
Standard (Peak Area) Blank (Peak
Area)
Area % of blank to
lowest calibrant
(Criteria = <30%)
Lowest calibrant
concentration ug/l
1
1.823
265.2>122.2
15055.6
373.6
2.48
5.0
2
1.823
265.2>122.2
15618.9
373.6
2.39
5.0
3
1.823
265.2>122.2
14562.7
373.6
2.57
5.0
4
1.823
265.2>122.2
15713.6
373.6
2.38
5.0
5
1.823
265.2>122.2
13737.0
373.6
2.72
5.0
6
1.823
265.2>122.2
13549.6
373.6
2.76
5.0
7
1.823
265.2>122.2
15270.3
373.6
2.45
5.0
8
1.823
265.2>122.2
15585.5
373.6
2.40
5.0
9
1.823
265.2>122.2
13433.8
373.6
2.78
5.0
10
1.823
265.2>122.2
12834.9
373.6
2.91
5.0
Mean
1.82
Mean
2.583
Concentration Analyte Retention
Time (Minutes)
265.2>81.3
Ion Ratio % Relative
Intensity
RT Difference
(criteria = ± 0.2
minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
1.823
9029.51718
2561
0.284
28.4
Pass
Confirmed
Calibration standard 2
1.823
16489.33435
4122
0.250
25.0
Pass
Confirmed
Calibration standard 3
1.823
165868.5171
42924
0.259
25.9
Pass
Confirmed
Calibration standard 4
1.823
374491.9321
100004
0.267
26.7
Pass
Confirmed
Calibration standard 5
1.823
768752.4174
196894
0.256
25.6
Pass
Confirmed
Calibration standard 6
1.823
1952246.075
495250
0.254
25.4
Pass
Confirmed
Mean
1.823
SRM 1849a-1
1.805
281221.43
75498.00
0.27
27
-0.018
Pass
Confirmed
SRM 1849a-2
1.823
287193.93
76109.00
0.27
27
0.000
Pass
Confirmed
SRM 1849a-3
1.823
305591.61
80795.00
0.26
26
0.000
Pass
Confirmed
SRM 1849a-4
1.823
304869.60
80035.00
0.26
26
0.000
Pass
Confirmed
SRM 1849a-5
1.823
293676.06
76317.00
0.26
26
0.000
Pass
Confirmed
SRM 1849a-6
1.823
287956.46
74177.00
0.26
26
0.000
Pass
Confirmed
Average ion ratio
0.26
Relative intensity (%
of base peak)
% Tolerance
plus or minus
Average intensity
26
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.18
>10-20
30
Upper limit
0.34
< 10
30
Page 5
AOAC- SPIFAN-2012-VITAMINS
Method Blank
TUV/BLR/F-360
LC-MS/MS
Manjunath K/Prasad BKVR
Specificity
B-Complex Vitamins
01.01.2015
Thiamine
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE