156 / 670
Validation Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Equiment
Analyst
Sample Blank No.
Retention Time (min)
m/z
Standard (Peak Area)
Blank (Peak Area)
Area % of blank to
lowest calibrant
(Criteria = <30%)
Lowest calibrant
concentration ug/l
1
2.097
170.2>152.1
46838.4
695.0
1.48
5.0
2
2.097
170.2>152.1
46494.7
695.0
1.49
5.0
3
2.097
170.2>152.1
47375.1
695.0
1.47
5.0
4
2.097
170.2>152.1
45064.5
695.0
1.54
5.0
5
2.097
170.2>152.1
47882.5
695.0
1.45
5.0
6
2.097
170.2>152.1
45994.3
695.0
1.51
5.0
7
2.097
170.2>152.1
45994.5
695.0
1.51
5.0
8
2.097
170.2>152.1
46518.0
695.0
1.49
5.0
9
2.097
170.2>152.1
47576.3
695.0
1.46
5.0
10
2.097
170.2>152.1
47071.2
695.0
1.48
5.0
Mean
2.10
Mean
1.489
Concentration Analyte Retention
Time (Minutes)
170.2>134.1
Ion Ratio
% Relative
Intensity
RT Difference
(criteria = ± 0.2
minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
2.843
52068.7609
37624
0.723
72.3
Pass
Confirmed
Calibration standard 2
2.843
93709.7676
68929
0.736
73.6
Pass
Confirmed
Calibration standard 3
2.843
445905.4511
333530
0.748
74.8
Pass
Confirmed
Calibration standard 4
2.843
801082.5817
609512
0.761
76.1
Pass
Confirmed
Calibration standard 5
2.843
1420134.093
1093000
0.770
77.0
Pass
Confirmed
Calibration standard 6
2.843
2771730.763
2129000
0.768
76.8
Pass
Confirmed
Mean
2.843
SRM 1849a-1
2.843
552992.20
431534.00
0.78
78.0
0.000
Pass
Confirmed
SRM 1849a-2
2.843
567524.26
444719.00
0.784
78.4
0.000
Pass
Confirmed
SRM 1849a-3
2.843
556656.83
439467.00
0.789
78.9
0.000
Pass
Confirmed
SRM 1849a-4
2.843
559318.84
446583.00
0.798
79.8
0.000
Pass
Confirmed
SRM 1849a-5
2.843
569052.39
447307.00
0.786
78.6
0.000
Pass
Confirmed
SRM 1849a-6
2.843
554270.79
444354.00
0.802
80.2
0.000
Pass
Confirmed
Average ion ratio
0.76
Relative intensity (% of
base peak)
% Tolerance plus or
minus
Average intensity
76
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.53
>10-20
30
Upper limit
0.99
< 10
30
Page 10
AOAC- SPIFAN-2012-VITAMINS
Method Blank
TUV/BLR/F-360
LC-MS/MS
Manjunath K/Prasad BKVR
Specificity
B-Complex Vitamins
01.01.2015
Pyridoxin
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE