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Title
VALIDATION OF B-COMPLEX VITAMINS
Test Parameters
Reference
AOAC -2012 - APPENDIX L, K
Products Selected
Infant Formula- Lactogen
Infant Formula - Cerelac
Eezee Paste
Fruit Juices
Parameters
No. of Runs
Replicates
Rule
Linearity/Calibration Fit
6
1
LOD
10
1
LOQ
10
1
Blank
6
1
RT Ratio = 0.95 to 1.05
Sample (1849a)
1
1
Ion Ratio = Refer Table
Repeatability (same analyst, same day)
Day-1
RSD & Horrat - Refer Table
5
1
Fresh Standards & Fresh
Reagents every batch
Reproducibility -(different -days/analysts)
Day-2
5
1
SRM 1849a
5
1
SRM 1849a
5
1
Recovery % - Refer Table
Page 3
AOAC Recommended Guideline for Stake holder Panel on Infant Formula and Adult
Nutritionals (SPIFAN)
Thiamine, Riboflavin, Niacinamide, Nicotinic acid,
Pantothenic acid, Pyridoxine, Biotin, Folic acid
SANCO-12571-2013 : Guidance document on analytical quality control and validation
procedures for pesticide residues analysis in food and feed.
Specificity
Precision
Accuracy (Trueness) -
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE