495 / 670
Contribution of minor folates to the total folate content of infant formula and
adult/pediatric nutritionals / F. Martin (NRC, AS)
03 Feb 2016
CONFIDENTIAL
ͼ This document may not be reproduced or disclosed to third parties without prior authorization
Page 9 / 13
Contribution of polyglutamate forms to total Vitamin B9 content
When analysing the same set of samples after an additional step with deconjugase, some minor folates were able to be quantified. Others (THF,
10CHOͲFA and 10MeFA) remained below the limit of quantification. Results are presented in the folowing table:
FA (μg/100 g RTF)
5MeTHF (μg/100 g RTF)
5,10MylͲTHF (μg/100 g
RTF)
5CHOͲTHF (μg/100 g
RTF)
Avg STEDEV CV Avg STEDEV CV
Avg STEDEV CV Avg STEDEV CV
MilkͲbased infant formula
19.1 1.80 9.4% 1.3
0.03 2.4% BLOQ
BLOQ
SoyͲbased infant formula
18.1 1.88 10.4% BLOQ
0.5
0.05 8.8% 1.7 0.00 0.2%
Infant formula powder, partially
hydrolyzed milkͲbased
15.7 0.16 1.0% BLOQ
BLOQ
BLOQ
Infant formula powder, partially
hydrolyzed soyͲbased
23.4 0.51 2.2% BLOQ
0.5
0.01 2.6% 1.6 0.01 0.4%
Adult nutritional ready to feed – high fat 78.9 1.92 2.4% BLOQ
BLOQ
BLOQ
Adult nutritional ready to feed – high
protein
58.0 2.63 4.5% BLOQ
BLOQ
BLOQ
MilkͲbased toddler Formula powder
16.0 0.04 0.2% 2.8
0.03 1.2% BLOQ
BLOQ
Milk based Infant formula powder
13.3 0.31 2.4% 0.3
0.00 0.4% BLOQ
BLOQ
Infant formula ready to feed – milkͲ
based
16.0 0.09 0.6% 0.6
0.00 0.3% BLOQ
BLOQ
SRM 1849a
22.3 1.01 4.5% 1.0
0.02 2.4% BLOQ
BLOQ
Child formula powder – milkͲbased
34.0 0.53 1.6% 1.8
0.02 1.1% BLOQ
BLOQ
LowͲFat adult nutritional powder
39.0 1.34 3.5% BLOQ
BLOQ
1.0 0.12 12%
Infant elemental powder
28.3 0.15 0.5% BLOQ
BLOQ
BLOQ
Infant formula powder – FOS/GOS based 10.0 0.66 6.6% 1.0
0.03 3.3% BLOQ
BLOQ
Contribution of the folic acid and other minor folates (including polyglutamates) after the deconjugase step are graphically represented in the
next page.
2013.13 (Fol-21) / (February 2016) - NOTES
FOR ERP USE ONLY
DO NOT DISTRIBUTE