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Contribution of minor folates to the total folate content of infant formula and
adult/pediatric nutritionals / F. Martin (NRC, AS)
03 Feb 2016
CONFIDENTIAL
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Page 11 / 13
When comparing results on compounds mentioned in the SMPR (folic acid, 5ͲmethylͲtetrahydrofolate
and its polyglutamates), the deconjugase step had a minor effect on the level of 5ͲmethylͲ
tetrahydrofolate in the tested products, as showed in the next table.
contribution of 5ͲMeͲTHF on total folates
(
per
SMPR)
without
deconjugase
with
deconjugase
Differenc
e
MilkͲbased infant formula
4.7%
6.7%
1.9%
SoyͲbased infant formula
0.0%
0.0%
0.0%
Infant formula powder, partially hydrolyzed milkͲ
based
0.0%
0.0%
0.0%
Infant formula powder, partially hydrolyzed soyͲbased
0.0%
0.0%
0.0%
Adult nutritional ready to feed – high fat
0.0%
0.0%
0.0%
Adult nutritional ready to feed – high protein
0.0%
0.0%
0.0%
MilkͲbased toddler Formula powder
14.2%
17.7%
3.5%
Milk based Infant formula powder
0.0%
2.6%
2.6%
Infant formula ready to feed – milkͲbased
2.8%
3.9%
1.1%
SRM 1849a
3.8%
4.6%
0.8%
Child formula powder – milkͲbased
3.6%
5.4%
1.9%
LowͲFat adult nutritional powder
0.0%
0.0%
0.0%
Infant elemental powder
0.0%
0.0%
0.0%
Infant formula powder – FOS/GOS based
7.6%
10.0%
2.4%
Average
1.0 %
On the fourteen products tested, the contribution of the polyglutamates forms of the 5ͲmethylͲ
tetrahydrofolate is only 1.0 % on the total vitamin B
9
content.
When analyzing the contribution of the minor folates compounds not mentioned in the SMPR, the
deconjugase treatment did indeed liberate minor folates, as illustrated in the table below. The two
other minor folates detected are the following compounds: 5,10ͲmethenylͲtetrahydrofolate (5Ͳ
10MylͲTHF), and 5ͲformylͲtetrahydrofolate (5CHOͲTHF).
2013.13 (Fol-21) / (February 2016) - NOTES
FOR ERP USE ONLY
DO NOT DISTRIBUTE