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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys
2
Contents
Sr.#
Title
Page#
1
Objective
3
2
Analyte Scope & Abbreviations used in the report
3
3
Matrix Scope
3
4
SPIFAN II Samples
3
5
Results
5
i)
Optimization of MS Parameters
5
ii)
Optimization of HPLC conditions
6
iii)
Evaluation of Linearity of the analyte response and calibration curves
10
iv)
Solvent Blank Evaluation
12
v)
Evaluation of Accuracy of the method using the reference materials
15
vi)
Evaluation of Precision of the method
16
6
Analysis of SPIFAN II Samples
20
a)
Sample Details
20
b)
Reconstitution of the powder SPIFAN samples
21
c)
Averages and %RSD of total folate analysis with conjugase
22
d)
Averages and %RSD of samples total folate analysis with no conjugase
24
e)
Comparison of results of total folate analysis with and without Conjugase
26
f)
Minor Folate contribution to total folates in analysis with and without conjugase
28
g)
% Spike Recovery of total folate in SPIFAN samples analyzed using conjugase
30
7
LOD and LOQ of the method (with conjugase)
33
8
Conclusions
34
9
References
34
2011.06/Fol-22 (February 2016)
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