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Silliker

Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys

2

Contents

Sr.#

Title

Page#

1

Objective

3

2

Analyte Scope & Abbreviations used in the report

3

3

Matrix Scope

3

4

SPIFAN II Samples

3

5

Results

5

i)

Optimization of MS Parameters

5

ii)

Optimization of HPLC conditions

6

iii)

Evaluation of Linearity of the analyte response and calibration curves

10

iv)

Solvent Blank Evaluation

12

v)

Evaluation of Accuracy of the method using the reference materials

15

vi)

Evaluation of Precision of the method

16

6

Analysis of SPIFAN II Samples

20

a)

Sample Details

20

b)

Reconstitution of the powder SPIFAN samples

21

c)

Averages and %RSD of total folate analysis with conjugase

22

d)

Averages and %RSD of samples total folate analysis with no conjugase

24

e)

Comparison of results of total folate analysis with and without Conjugase

26

f)

Minor Folate contribution to total folates in analysis with and without conjugase

28

g)

% Spike Recovery of total folate in SPIFAN samples analyzed using conjugase

30

7

LOD and LOQ of the method (with conjugase)

33

8

Conclusions

34

9

References

34

2011.06/Fol-22 (February 2016)

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