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Fonterra Co-operative Group

Page 15

Figure 7: Vitamin D

3

MRM chromatogram of milk-based infant formula sample

Figure 8: Vitamin D

2

MRM chromatogram of milk-based infant formula sample

Mass Spectrometry

During method development, product ion scans of the fragmentation of a standard of vitamin D

3

-PTAD and

vitamin D

2

-PTAD [M+H]

+

ions were performed (Figures 9

10). Product ions (298 and 280

m/z

) were

identified as being suitable candidates for quantifier and qualifier ions for both vitamin D

2

and vitamin D

3

.

The method was optimised to enhance the signal of the transitions 572.2

Æ

298 and 572.2

Æ

280 for

vitamin D

2

and 560.2

Æ

298 and 560.2

Æ

280 for vitamin D

3

.

VitD-18 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE