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Fonterra Co-operative Group
Page 19
Table A4: Data vitamin D
3
linearity experiment 1
Concentration
(1)
NLD
3
(ng mL
-1
)
Concentration
Ratio
(2)
Peak Area Ratio
Back-calculated
Conc. Ratio
Variance
(%)
0.5
0.14
0.20
0.18
25.0
2.3
0.71
1.01
0.75
5.6
11.6
3.53
4.93
3.50
-1.0
23.2
7.06
9.89
6.97
-1.3
57.9
17.66
25.22
17.73
0.4
92.6
28.26
40.18
28.24
-0.1
Regression Equation
y = 1.4247x
–
0.0479
(1)
Concentration of non-labelled vitamin D
3
in calibration standards
2)
Ratio of concentration of non-labelled vitamin D
3
to isotope-labelled vitamin D
3
in calibration standards
Table A5: Data vitamin D
3
linearity experiment 2
Concentration
(1)
NLD
3
(ng mL
-1
)
Concentration
Ratio
(2)
Peak Area Ratio
Back-calculated
Conc. Ratio
Variance
(%)
0.5
0.14
0.21
0.22
55.8
2.3
0.70
0.96
0.71
0.4
11.5
3.52
5.19
3.45
-1.8
23.1
7.04
10.13
6.67
-5.2
57.7
17.59
27.92
18.25
3.8
92.3
28.14
42.65
27.83
-1.1
Regression Equation
y = 1.537x + -0.1227
(1)
Concentration of non-labelled vitamin D
3
in calibration standards
(2)
Ratio of concentration of non-labelled vitamin D
3
to isotope-labelled vitamin D
3
in calibration standards
Table 7: Data vitamin D
3
linearity experiment 3
Concentration
(1)
NLD
3
(ng mL
-1
)
Concentration
Ratio
(2)
Peak Area Ratio
Back-calculated
Conc. Ratio
Variance
(%)
0.5
0.14
0.20
0.26
79.4
2.3
0.71
0.97
0.80
11.9
11.7
3.56
4.69
3.44
-3.5
23.4
7.12
9.76
7.04
-1.3
58.4
17.81
24.87
17.76
-0.3
93.4
28.50
40.11
28.57
0.2
Regression Equation
y = 1.4096x
–
0.1562
(1)
Concentration of non-labelled vitamin D
3
in calibration standards
(2)
Ratio of concentration of non-labelled vitamin D
3
to isotope-labelled vitamin D
3
in calibration standards
VitD-18 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE