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Fonterra Co-operative Group

Page 16

Figure 9: Product ion scan of vitamin D

2

-PTAD standard; [M+H]

+

= 572.2 m/z

Figure 10: Product ion scan of vitamin D3-PTAD standard; [M+H]

+

= 560.2 m/z

Pre-Vitamin D

The separate measurement of pre-vitamin D was explored as part of initial method proof of concept [6]. It

was decided that a separate measurement of pre-vitamin D in this method was not needed. As discussed,

there are a number of practical reasons for not including a separate measurement of pre-vitamin D: (1) the

VitD-18 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE