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Fonterra Co-operative Group

Page 18

Appendix A: Data—Linearity

Table A1: Data vitamin D

2

linearity experiment 1

Concentration

(1)

NLD

2

(ng mL

-1

)

Concentration

Ratio

(2)

Peak Area Ratio

Back-calculated

Conc. Ratio

Variance

(%)

0.3

0.04

0.03

0.03

-29.0

1.5

0.18

0.17

0.18

-0.7

7.6

0.90

0.81

0.89

-0.9

15.1

1.80

1.64

1.81

1.0

37.9

4.49

4.06

4.50

0.3

60.6

7.18

6.45

7.17

-0.2

Regression Equation

y = 0.8987x + 0.0105

(1)

Concentration of non-labelled vitamin D

2

in calibration standards

(2)

Ratio of concentration of non-labelled vitamin D

2

to isotope-labelled vitamin D

2

in calibration standards

Table A2: Data vitamin D

2

linearity experiment 2

Concentration

(1)

NLD

2

(ng mL

-1

)

Concentration

Ratio

(2)

Peak Area Ratio

Back-calculated

Conc. Ratio

Variance

(%)

0.3

0.04

0.03

0.03

-30.1

1.5

0.18

0.15

0.16

-8.5

7.6

0.90

0.78

0.89

-0.4

15.1

1.79

1.58

1.82

1.3

37.8

4.48

3.90

4.51

0.6

60.5

7.18

6.17

7.15

-0.3

Regression Equation

y = 0.8609x + 0.0117

(1)

Concentration of non-labelled vitamin D

2

in calibration standards

(2)

Ratio of concentration of non-labelled vitamin D

2

to isotope-labelled vitamin D

2

in calibration standards

Table A3: Data vitamin D

2

linearity experiment 3

Concentration

(1)

NLD

2

(ng mL

-1

)

Concentration

Ratio

(2)

Peak Area Ratio

Back-calculated

Conc. Ratio

Variance

(%)

0.3

0.03

0.03

0.02

-34.4

1.4

0.17

0.15

0.16

-5.8

7.2

0.86

0.74

0.84

-1.8

14.4

1.71

1.50

1.72

0.6

36.1

4.28

3.77

4.35

1.7

57.8

6.84

5.88

6.80

-0.7

Regression Equation

y = 0.8624x + 0.0137

(1)

Concentration of non-labelled vitamin D

2

in calibration standards

(2)

Ratio of concentration of non-labelled vitamin D

2

to isotope-labelled vitamin D

2

in calibration standards

VitD-18 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE