SPADA Working Groups ~ April Meeting Book

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STAKEHOLDER PANEL ON
Agent detection
Background & Fitness for Purpose
Coxiella burnetii
James E. Samuel, PhD
Professor and Chair, TAMHSC
February 3, 2015
 Legionellales, obligate intracellular parasite
 G ti ith LPS Ph I d Ph II
Baca et al. 1984
ram nega ve w : ase an ase
 Metabolically active axenically, esp. at lower pH
 Life cycle: LCV and SCV
 Acute (>50% seroconvert-asymptomatic) and chronic infectious
disease
 Broad zoonotic reservoir; high seropositivity rate
 Typical route of infection via aerosol of contaminated soils
 R li t i “ d l d” h l lik l ep ca es n a remo e e p ago ysosome- e vacou e
 Genomic predictions * : ~2150 ORFs
 Complete TCA, various aa auxotrophs
 Large group of transporters
 Proteomic skew to high pI
 Complete Type 4 secretion element
 >200 genes with single/point mutation “pseudogene”
*Seshadri et al. PNAS 2003