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Mouse sublethal challenge model
Infected
mice:IPWild type mice relatively resistant to clinical disease but readily
colonized: splenomegaly correlates with bacterial load
Aerosol and oral challenges models developed for virulence
and protection studies
Zhang
et al.
IAI 2004
1 4
0
0.2
0.4
0.6
0.8
1
1.2
.
0.E+00 1.E+05 1.E+06 1.E+07 1.E+08 1.E+09
C. burnetii
challenge dose (Log
10
)
Spleen Weight
Spleen Weight
1 0
*
*=p<0.05
**=p<0.01
Whole Spleen Cb Copy #
1 0 10
7
Mouse Infection-derived Immunity: IP Challenge
1 2 3 4 5 6 7 8
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
.
Average Spleen Weight (g)
**
**
*
1 2 3 4 5 6 7 8
1.0_10
3
1.0_10
4
1.0_10
5
1.0_10
6
. _
Average Genome Copy # per
Whole Spleen
1
Negative control
2
10
6
Cb inf (3 wk)
3
10
6
Cb inf (3 wk), Doxy (2 wk), rest (1 wk)
4
10
6
Cb inf (6 wk)
5
10
6
Cb inf (3 wk), Doxy (2 wk), rest (1 wk), 10
7
Cb inf (2wk)
6
10
6
Cb inf (6 wk), 10
7
Cb inf (2wk)
7
Non-inf (6 wk), 10
7
Cb inf (2wk)
8
pI vax (6 wk), 10
7
Cb inf (2wk)
Group
Group