121 / 134
C. burnetii
phylogenetic organization
SNP and VNTR based trees for 25
worldwide isolates of
Coxiella
burnetii.
Geographical distribution
shown below.
Pearson, Keim et al. ASM2005
Glazunova
et al.
EID 2005
C. burnetii
– Existing PCR assays
•
Purpose: Detect
C. burnetii
DNA in
clinical and environmental samples
–
Environmental samples, milk, soil, animal
Tissues, air sampling
–
Human blood samples
•
Most common targets:
IS1111, mulit‐copy IS element
–
Range in copy number among isolates (~20)
Com‐1: encodes outer membrane (DsbA/C) protein
–
Highly conserved among isolates
•
qPCR method
–
Brennan et al (IAI 2003)
–
Sensitivity approaches one genome equivalent