Methods to be Reviewed by OMB in 2016

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12.Room Light Shields: A.L.P. Protect-A-Lamp, UV cutoff at 460 nm, or equivalent.
13.Graduated cylinders: various sizes, including: 10, 100, 500, and 1000 mL.
14.Beakers: various sizes, including: 100, 200, 400, 600, 1000, and 2000 mL.
15.Volumetric flasks: various sizes, including: 10, 25, 50, 100, 250, and 2000 mL.
16.Mobile phase bottles: glass, various sizes, including: 250, 500, 1000, and 2000 mL .
17.Disposable, plastic Pasteur pipettes.
18.Amber bottles: volume capacity of 50 and 100 mL (for stock standard storage).
19.Weighing vessels: various, including disposable weighing boats and glass weighing funnels.
20.Positive displacement pipettes: Gilson Microman - 10, 100, 250, and 1000 µL; Part #’s F148501,
F148504, F148505, and F148506.
21.Positive displacement pipette tips: Gilson Capillary Piston - 10, 100, 250, and 1000 µL; Part #’s
F148312, F148314, F148014, and F148560.
22.Plastic syringes, 3 mL.
23.Syringe filters, PTFE 0.45 µm syringe filters: Acrodisc 25 mm, or equivalent.
24.50 mL self-standing, plastic centrifuge tubes: Superior Scientific, Ltd., or equivalent.
25.Autosampler vials: Waters autosampler vials; 9 mm amber with screw top 12x32 mm pre-split
PTFE-silicon septa; Waters Part # 186000847C, or equivalent.
26.Teflon coated magnetic stir bars.
C. Reagents
1. Nicotinamide: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog #
1462006. Store as indicated on label.
2. Niacin (nicotinic acid): USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot;
Catalog # 1461003. Stored as indicated on label.
3. Pyridoxine hydrochloride: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot;
Catalog # 1587001. Store in desiccator protected from white light. Dry according to
manufacturer’s instructions prior to use.
4. Riboflavin: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog #
1603006. Store in desiccator protected from white light. Dry according to manufacturer’s
instructions prior to use.
5. Thiamine hydrochloride: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot;
Catalog # 1656002. Store in desiccator protected from white light. Measure the moisture
content of the powder prior to use.
6. Pyridoxamine dihydrochloride: Fluka Analytical Standard, catalog# P9380.
7. Pyridoxal hydrochloride: Sigma, catalog# P9130.
8. 2H4-Nicotinamide: CDN Isotopes; Catalog # D-3457.
9. 2H4-Nicotinic acid: CDN Isotopes; Catalog # D-4368.
Candidates for 2016 Method of the Year
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