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10.
13
C
4
-Pyridoxine: pyridoxine:HCl (4,5-bis(hydroxymethyl)-
13
C
4
); Cambridge Isotope Laboratory;
Catalog # CLM-7563.
11.
2
H
3
-Pyridoxal: IsoSciences; Catalog # 7098.
12.
2
H
3
-Pyridoxamine: IsoSciences; Catalog # 7099.
13.
13
C
4
-Thiamine chloride; IsoSciences; Catalog # 9209.
14.
13
C
4
,
15
N
2
-Riboflavin: IsoSciences, Catalog# 7072.
15.Acid phosphatase, type II from potato, 0.5 – 3.0 U/mg: Sigma, catalog# P3752.
16.Papain from Carica papaya, ≥ 3 U/mg: Sigma, catalog# 76220.
17.α–amylase from aspergillus oryzae, 150 U/mg: Sigma, catalog# A9857.
18.Hydrochloric acid concentrated (12M); ACS grade, or equivalent.
19.Ammonium formate: for mass spectrometry (≥99.0%), Fluka 70221 or equivalent.
20.Glacial acetic acid: Sigma ACS Reagent Grade, or equivalent.
21.Formic acid: Sigma ACS Reagent Grade, or equivalent.
22.Laboratory Water: 18.0 MΩ, < 10 ppb TOC, or equivalent.
23.Methanol: Fisher LC-MS/MS Optima grade or EMD Omni-Solve LC-MS grade.
24.Ethylenediaminetetracetic acid, disodium salt dihydrate (EDTA): ACS grade (99-101%), or
equivalent.
25.Potassium phosphate dibasic: ACS grade (>98%), or equivalent.
26.meta-Phosphoric acid: ACS grade (33.5-36.5%), or equivalent.
27.pH4.0, 7.0, and 10.0 buffer solutions for pH meter calibration.
28.Phosphoric acid, 85%, ACS grade, or equivalent.
29.Potassium hydroxide, 40%, ACS grade, or equivalent.
D.
Standard and Solution Preparation
1.
Mobile Phase A: 20 mM ammonium formate in water - Using a graduated cylinder, transfer
500 mL laboratory water to a mobile phase reservoir. Add 0.631 g of ammonium formate and
mix well. Scale as needed. Expiration: 3 days.
2.
Mobile Phase B: Methanol.
3.
Weak needle wash: 10% methanol in water, expiration 3 mo.
4.
Strong needle wash: Methanol.
5.
50 mM ammonium formate - Using a graduated cylinder, transfer 1400 mL laboratory water to
an appropriate reservoir. Add 4.41 g of ammonium formate and mix well. 1400 mL is
adequate for 6 working standards and 32 samples. Scale as needed. Expiration: 3 days.
6.
Enzyme Cocktail - Using a graduated cylinder, transfer 200 mL 50 mM ammonium formate
buffer to an appropriate reservoir. Add 200 ± 10 mg acid phosphatase, 80 ± 5 mg α-amylase,
Candidates for 2016 Method of the Year
190