(j)
PTFESyringeFilter
(k)
Volumetric Flasks (ClassA)
(l)
Autopipettor 1mL
(m)
Graduatedmeasuring cylinders (ClassA)
(n)
Autosampler vials, glass inserts and caps
(o)
Disposable centrifuge tubes
(p)
AutoclaveUnit (Astell)
(q)
Heating block set at 100±5
⁰
C (Environmental ExpressUSA)
ChemicalsandReagents
(a)
MilliQWater (EMDMillipore, Billerica, MA)
(b)
SodiumDihydrogenPhosphateDihydrate (Merck KGaA, Germany)
(c)
DisodiumHydrogenPhosphateDihydrate (Merck KGaA, Germany)
(d)
SodiumHydroxide (Merck KGaA, Germany)
(e)
SodiumChloride (Merck KGaA, Germany)
(f)
PotassiumDihydrogenPhosphate (Merck KGaA, Germany)
(g)
Methanol, HPLCgrade (MerckKGaA, Germany)
(h)
Acetonitrile, HPLCgrade (MerckKGaA, Germany)
(i)
Ortho-phosphoric acid, 85% (Fisher Scientific, UK)
(j)
Biotin, purity 99%: SigmaB4501or equivalent
(k)
Phosphatebuffered saline (PBS) pH7.4: Dissolve 2.38g of disodium hydrogen
phosphate, 0.19g of potassium dihydrogen phosphate and8.0g of sodium chloride inwater.
Dilute to1000mL and adjust pH if necessary.
(l)
MobilePhase: 0.1%Phosphoric acid: Acetonitrile (85:15%v/v)
(m)
2MSodiumHydroxide:Weigh80g of sodiumhydroxide into a 1L volumetric flask,
dissolve inwater andmakeup tomark.
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