SimultaneousDetermination of SevenWater SolubleVitamins inProducts byLC-MS/MS
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Table 2. Method performance requirements for the analysis of folic acid
a
Analytical range
0.50–300
b
Limit of detection (LOD)
≤0.10
b
Limit of quantitation (LOQ)
≤0.50
b
Repeatability (RSDr)
0.50
b
≤11%
21.5
b
≤7%
43.0
b
64.0
b
85.0
b
Recovery
0.5
90–110%
21.5
b
43.0
b
64.0
b
85.0
b
Reproducibility (RSDR)
0.5
b
≤32%
21.5
b
≤16%
43.0
b
64.0
b
85.0
b
Note: Thismethod as defined determines fortified folic acid andnot total folate
Table 3. Methodperformance requirements for the analysis of pantothenic acid
a
Analytical range
50–2300
b
Limit of quantitation (LOQ)
≤50
b
Repeatability (RSDr)
50–2300
b
≤5%
Recovery
90 to 110% ofmean spiked recovery
over the range of the assay
Reproducibility (RSDR)
50–2300
b
≤15%
a
Concentrations apply to: (
1
) “ready-to-feed” liquids “as is”;
(
2
) reconstituted powders (25g into 200 gwater); and (
3
) liquid concentrates
diluted 1:1 byweight.
b
mcg/100 g reconstituted final product.
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