Table 1 for sampleweight and loading volume for different biotin range
Product (µg/100g)
SamplePreparation
Conc (µg/100mL)
Min
Max
Weight (g)
Volume (mL) Load (mL) Final (mL)
Min
Max
0.1
1.0
20
100
50
1
1
10
1.0
5.0
10
100
10
1
1
5
5.0
50.0
2
100
10
1
1
10
50.0
100.0
1
100
10
1
5
10
100.0
250.0
0.5
100
5
1
2.5
6.25
Table 2: Precision (Repeatability)
Products
Biotin (µg/100g)
%RSD
%RSD
HARRATr
Sample1 -Day1
22.2
1.13
3.97
0.5
Sample1 -Day2
21.9
0.52
Sample1 -Day1
12.6
1.72
5.28
0.5
Sample1 -Day2
12.6
2.41
Table 3: Recovery
CalibrationRange
Added (µg/100g)
Found (µg/100g)
Recovery (%)
Low
3.07
2.89
94
3.07
2.85
93
3.02
2.85
94
Medium
5.04
5.20
103
5.10
5.00
98
5.11
5.10
100
High
10.24
10.05
98
10.24
9.94
97
10.23
10.65
104
BIO-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
