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B
ird
et al
.:
J
ournal of
aoaC i
nternational
V
ol
.
99, n
o
.
4, 2016
993
Raw Ground Beef (73% Lean; 325 g Test Portions)
Raw ground beef test portions were inoculated at a low
and high level and analyzed (Table 3) for the detection of
Salmonella
spp. Uninoculated controls were included in each
analysis. Laboratories 7 and 10 did not participate in the
evaluation of this matrix. Laboratory 5 received test portions
but was unable to conduct the analysis and, therefore, no data
were submitted. All other laboratories submitted a full set of
data for each method. The MPN levels obtained for this test
portion were 0.67 CFU/test portion (95% confidence interval,
0.53–0.83) for the low inoculum level and 4.76 CFU/test
portion (95% confidence interval, 3.41–6.65) for the high
inoculum level.
For the low inoculum level, 83 of 156 test portions (POD
CP
of 0.53) were reported as presumptive positive by the 3M MDA
Table 4.
Individual collaborator results for creamy peanut butter
High level test portions
Low level test portions
Uninoculated test portions
1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 5 6 7 8 9 10 11 12
3M MDA 2 –
Salmonella
Laboratory
a
1
+ + + + + + + + + + + + + — — — — — — + + + + + — — — — — — — — — — — —
2
+ + + + + + + + + + + + + + + + — — + — — + + + — — — — — — — — — — — —
3
b
+ + + + + + + + + + + + — — — — +
c
+ — + + + + — — — — — — — — + — +
c
— —
4
+ + + + + + + + + + + + + + — — — + — + — + + — — — — — — — — — — — — —
5
NA
d
NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA
6
+ + + + + + + + + + + + + + + — + + — + + — -
e
+ — — — — — — — — — — — —
7
b
+ + + + + + + + + + + + + -
e
+ — + -
e
+ + + + — + — — — — — — — -
e
— — — —
8
+ + + + + + + + + + + + + — — + — — + — + + + — — — — — — — — — — — — —
9
+ + + + + + + + + + + + — + + + + — + + — + — — — — — — — — — — — — — —
10
+ + + + + + + + + + + + — — + — — + + + — + + + — — — — — — — — — — — —
11
NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA
12
+ + + + + + + + + + + + — + + + — — — — — — + + — — — — — — — — — — — —
13
+ + + + + + + + + + + + + + — + — + + + + — + -
e
— — — — — — -
e
-
e
— — — —
14
+ + + + + + + + + + + + + + + — — — + + + + — — — — — — — — — — — — — —
15
+ + + + + + + + + + + + + + — — + + — + + — + + — — — — — — — — — — — —
16
NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA
FDA BAM Chapter 5
1
+ + + + + + + + + + + + — — — — + + + — + + + — — — — — — — — — — — — —
2
+ + + + + + + + + + + + — + — — + + — — — + + + — — — — — — — — — — — —
3
b
+ + + + + + + + + + + + + + — — + — — + — — — + — — — — — — — — — — — —
4
+ + + + + + + + + + + + + — — + — — + — — — + + — — — — — — — — — — — —
5
NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA
6
+ + + + + + + + + + + + + + — — — + — + + + + + — — — — — — — — — — — —
7
b
+ + + + + + + + + + + + — + + — + — — — + + + + — — — — — — — — — — — —
8
+ + + + + + + + + + + + — — + + + — — — — — + + — — — — — — — — — — — —
9
+ + + + + + + + + + + + + + + + + + + — — — + — — — — — — — — — — — — —
10
+ + + + + + + + + + + + + + — — + — — — + — + — — — — — — — — — — — — —
11
NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA
12
+ + + + + + + + + + + + — — + — + — + + + — + — — — — — — — — — — — — —
13
+ + + + + + + + + + + + — — — — — + + — + + — + — — — — — — — — — — — —
14
+ + + + + + + + + + + + + — + — + + + + + — + — — — — — — — — — — — — —
15
+ + + + + + + + + + + + — + — — + + + + — + — — — — — — — — — — — — — —
16
NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA
a
+ =
Salmonella
spp. were detected in samples; - =
Salmonella
spp. were not detected in sample.
b
Results were not used in statistical analysis due to laboratory error.
c
Sample was presumptive negative on 3M MDA 2 –
Salmonella
but confirmed positive indicating a false negative result.
d
NA = Laboratory did not participate or submit data for the matrix.
e
Sample was presumptive positive on 3M MDA 2 –
Salmonella
but confirmed negative indicating a false positive result.