COMMENT PARAMETERS
USER COMMENTS
AOAC OMA
2011.20
Method Name/Manuscript Title
5’ Mononucleotides in Infant Formula and Adult Nutritionals by liquid
chromatography
Manuscript Reference
In your experience using the method, does the
method perform according to the method’s
applicability as written?
This method did not meet the ranges listed for SRM 1849a. CMP and
GMP were both high out of spec.
Also, results near the LOQ showed poor chromatography.
In your experience with the method, is there
any safety concerns identified while using or
regarding use of the method?
No safety concerns were noted.
Do you have any information that supports
regarding the reproducibility of the method as
written: If so, please specify and submit
information?
Reproducibility meets the SMPR however it is on the high side at low
levels.
Please attach any documentation to support
your feedback, if available.
See 2011.20 chromatogram
Based on your experience with the method,
are there any recommended changes to the
AOAC First Action Method as written?
This method has the following limitations:
Heavy and time consuming sample preparation ‐ Numerous
interferences leading to difficulties with integration.
Although the SMPR does not list a minimum time for results, this
method’s time of analysis significant when performing the required
SPE step. When dealing with unknown samples analysts would not
know if the sample requires ultra‐centrifugation until loaded onto the
SPE cartridge. At that time the sample would need to be repeated.
In addition to these disadvantages, the method was not applicable as
it is to some products such as due to Interferences in the LC‐DAD
chromatograms.
The SMPR states “It would also be desirable to measure the
corresponding nucleosides” This candidate method cannot be
applied to nucleosides. However, further investigation might allow
2011.21 to adapt the method and include them in the future.
At this time I would not recommend changes to this method. I would
recommend that 2011.21 be re‐reviewed and subjected to MLT for
comparison to this methods data.
At this time I do not see this method having dispute method criteria.
AOAC INTERNATIONAL
Stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN)
