COMMENT PARAMETERS
USER COMMENTS
AOAC OMA
2012.13
Method Name/Manuscript Title
Labeled Fatty Acids Content in Milk Products and Infant Formula by
GC
Manuscript Reference
In your experience using the method, does the
method perform according to the method’s
applicability as written?
Sample preparation is found to be bit complicated with the use of
sodium ethoxide.
Chromatogram reveals a poor resolution of trans fatty acids.
Method recommends the cold fat extraction for product
supplemented with PUFA having fish oil. Profile may change due to
incomplete fat extraction.
Naturally occurring trans‐fatty acid‐ Vaccenic acid found in the fat of
ruminants and in dairy products such as milk, butter may interfere.
As DHA and EPA content is very low in health drinks the recovery may
affect.
In your experience with the method, is there
any safety concerns identified while using or
regarding use of the method?
None
Do you have any information that supports
regarding the reproducibility of the method as
written: If so, please specify and submit
information?
‐
Please attach any documentation to support
your feedback, if available.
Based on your experience with the method,
are there any recommended changes to the
AOAC First Action Method as written?
Need simple sample preparation procedure.
AOAC INTERNATIONAL
Stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN)
