AOAC RI ERP Final Action Recommendations - BOOK

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2. Upon receipt of shipment, remove sample portion labeled “Temperature Control”, open
sample container and insert thermometer (alternatively, an infrared thermometer can be used).
Record temperature (°C) on Sample Receipt Confirmation Form provided in sample package and
fax or email to Patrick Bird at 513-471-5600 or
BAX® System Real-Time PCR Assay for Salmonella Collaborative Study Materials
Materials supplied by DuPont
All BAX® System Real-Time PCR Assay for Salmonella detection kits (includes BAX® System lysis
buffer – Two bottles of 12 mL each, BAX® System protease – One vial of 400 µL, BAX® System PCR
tubes – 12 strips of 8 tubes containing one PCR tablet per tube.), BAX® System Q7 cycler/detector
with computer workstation, BAX® System application software and BAX®MP Media
Materials provided by Q Laboratories, Inc., Coordinating Laboratory
1. Standard Methods Agar for background APC of hot dogs and orange juice
Enrichment
2. Universal Pre-enrichment broth
3. BPW
4. RV-R10 broth for USDA reference method
5. Individual Ingredients to prepare FDA RV
6. TT Broth (Hajna formulation) for USDA reference method
7. TT Broth for FDA reference method
Selective agar
8. Hektoen Agar
9. XLD Agar
10. Bismuth Sulfite
11. XLT4 Agar
12. Brilliant Green Sulfa Agar
Confirmation
13. TSI
14. LIA
15. BHI
16. API 20E or VITEK GN cards
17. O and H serotyping supplies
Collaborative Study Approved Protocol
Expert Review Panel Use Only