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25

regrowth

NA

5

5

1.0

(0.57, 1.0)

5

1.0

(0.57, 1.0)

0

(-0.45, 0.45)

Plastic

surfaces

LB/4”X4”

sponge/18 hr +/-

regrowth

S

. Mbandaka

(DD13240)

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

NA

20

13

0.65

(0.43, 0.82)

13

0.65

(0.43, 0.82)

0

(-0.14, 0.14)

NA

5

5

1.0

(0.57, 1.0)

5

1.0

(0.57, 1.0)

0

(-0.45, 0.45)

Tile surfaces

BPW/4”X4”

sponge 18 hr +/-

regrowth

S

. Lexington

(DD13068)

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

NA

20

6

0.3

(0.15, 0.52)

6

0.3

(0.15, 0.52)

0

(-0.14, 0.14)

NA

5

5

1.0

(0.57, 1.0)

5

1.0

(0.57, 1.0)

0

(-0.45, 0.45)

Tile surfaces

LB/4”X4” sponge

18 hr +/- regrowth

S

. Lexington

(DD13068)

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

NA

20

9

0.45

(0.26, 0.66)

9

0.45

(0.26, 0.66)

0

(-0.14, 0.14)

NA

5

5

1.0

(0.57, 1.0)

5

1.0

(0.57, 1.0)

0

(-0.14, 0.14)

White

Pepper

TSB/25 g/22 hrs

S

. Newport

(DD13079)

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

1.3 (0.81, 2.2)

20

14

0.7

(0.48, 0.85)

14

0.7

(0.48, 0.85)

0

(-0.14, 0.14)

26 (20, 44)

5

5

1.0

(0.57, 1.0)

5

1.0

(0.57, 1.0)

0

(-0.14, 0.14)

Ground Beef

with Soy

MLG

mTSB/325g/22 hr

S

. Typhimurium

(DD1467)

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.14, 0.14)

1.0 (0.74, 1.4)

60

39

0.65

(0.52, 0.76)

39

0.65

(0.52, 0.76)

0

(-0.049, 0.049)

6.0 (4.4, 8.4)

5

5

1.0

(0.57, 1.0)

5

1.0

(0.57, 1.0)

0

(-0.45, 0.45)

Shell Eggs

MP/1000 g/48 hrs

S. Enteritidis

(ATCC13706)

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.14, 0.14)

1.6 (0.93, 3.1)

20

17

0.85

(0.64, 0.95)

14

0.70

(0.48, 0.85)

0.15

(-0.11, 0.39)

a

MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator, with 95% confidence interval

b

N = Number of test potions

c

x = Number of positive test portions

d

POD

C

=

Candidate method presumptive positive outcomes confirmed positive

e

POD

R

= Reference method confirmed positive outcomes divided by the total number of trials

f

dPOD

C

= Difference between the candidate method and reference method POD values

g

95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

Collaborative Study Approved Protocol

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