AOAC RI ERP Final Action Recommendations - BOOK

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Table 4. Data Summary – Test method vs Reference Method
Matrix
Enrichment
Media/Sample
Size/Enrichment
Time
Strain
MPNa/Test Portion
(cfu / test portion
for surface testing)
(95% CI)
Nb
BAX® Method Reference Method
dPODc
f 95% CIg
xc PODC
d 95% CI x PODR
e 95% CI
Ground Beef TSB+n/375g/ 24
hr
S. Heidelberg
DD13017
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.37 (0.20, 0.59) 20 5 0.25 (0.11, 0.4) 4 0.20 (0.08, 0.42) 0.05 (-0.21, 0.30)
Ground Beef BPW/25g/ 20 hr S. Heidelberg
DD13017
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.37 (0.20, 0.59) 20 4 0.2 (0.08, 0.42) 4 0.2 (0.08, 0.42) 0 (-0.14, 0.14)
Chicken
Rinse
BPW/30ml/16 and
22 hr
Naturally
Occurring NA 20 11 0.55 (0.34, 0.74) 11 0.55 (0.34, 0.74) 0 (-0.14, 0.14)
Cream
Cheese
MP/25g/12 and
24 hr
S. Typhimurium
DD586
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.20 (0.077, 0.39) 20 2 0.1 (0.03, 0.30) 5 0.25 (0.11, 0.47) -0.15 (-0.38, 0.022)
Cream
Cheese LB/25g/24 hr
S. Typhimurium
DD586
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.20 (0.077, 0.39) 20 5 0.25 (0.11, 0.47) 5 0.25 (0.11, 0.47) 0 (-0.14, 0.14)
Lettuce MP/25g/12 and
24 hr
S. Newport
DD1261
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.40 (0.22, 0.64) 20 7 0.35 (0.18-0.57) 4 0.20 (0.08, 0.42) 0.15 (-0.13, 0.40)
4.3 (2.5, 8.2) 5 5 1.0 (0.57, 1.0) 5 1.0 (0.57, 1.0) 0 (-0.45, 0.45)
Lettuce LB/25g/24 hr
S. Newport
DD1261
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.40 (0.22, 0.64) 20 4 0.2 (0.08, 0.42) 4 0.2 (0.08, 0.42) 0 (-0.14, 0.14)
4.3 (2.5, 8.2) 5 5 1.0 (0.57, 1.0) 5 1.0 (0.57, 1.0) 0 (-0.45, 0.45)
Lettuce LB/25g/24 hr +
regrowth
S. Newport
DD1261
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.40 (0.22, 0.64) 20 3 0.15 (0.05, 0.36) 4 0.2 (0.08, 0.42) -0.05 (-0.29, 0.19)
4.3 (2.5, 8.2) 5 5 1.0 (0.57, 1.0) 5 1.0 (0.57, 1.0) 0 (-0.45, 0.45)
Dry Pet Food BPW/375g/24 +
regrowth
S. Tennessee
DD13062
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.29 (0.13, 0.53) 20 5 0.25 (0.11, 0.47) 5 0.25 (0.11, 0.47) 0 (-0.14, 0.14)
Dry Pet Food LB/375g/24 +
regrowth
S. Tennessee
DD13062
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.29 (0.13, 0.53) 20 5 0.25 (0.11, 0.47) 5 0.25 (0.11, 0.47) 0 (-0.14, 0.14)
Collaborative Study Approved Protocol
Expert Review Panel Use Only