AOAC RI ERP Final Action Recommendations - BOOK

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Table 3. Data Summary – Test Method vs Confirmed Results From Test Method Enrichment
Matrix
Enrichment
Media/
Sample Size/
Enrichment
Time
Strain
MPNa/Test
Portion (cfu/test
portion for
surface testing)
Nb
BAX® Presumptive BAX® Confirmed
dPODCP
f 95% CIg
xc PODCP
d 95% CI x PODCC
e 95% CI
Ground Beef TSB+n/375g/24 hr
S. Heidelberg
DD13017
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.37 (0.20, 0.59) 20 5 0.25 (0.11, 0.47) 5 0.25 (0.11, 0.47) 0 (-0.14, 0.14)
Ground Beef
BPW/25g/
20 hr
S. Heidelberg
DD13017
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.37 (0.20, 0.59) 20 4 0.2 (0.08, 0.42) 4 0.2 (0.08, 0.42) 0 (-0.14, 0.14)
Chicken
Rinse
BPW/30ml/16
and 22 hr
Naturally
Occurring NA 20 11 0.55 (0.34, 0.74) 11 0.55 (0.34, 0.74) 0 (-0.14, 0.14)
Cream
Cheese
MP/25g/12
and 24 hr
S.
Typhimurium
DD586
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.20 (0.077, 0.39) 20 2 0.1 (0.028, 0.30) 2 0.1 (0.028, 0.30) 0 (-0.14, 0.14)
Cream
Cheese LB/25g/24 hr
S.
Typhimurium
DD586
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.20 (0.077, 0.39) 20 5 0.25 (0.11, 0.47) 5 0.25 (0.11, 0.47) 0 (-0.14, 0.14)
Lettuce MP/25g/12
and 24 hr
S. Newport
DD1261
0.00 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.40 (0.22, 0.64) 20 4 0.20 0.08-0.42 4 0.2 0.08-0.42 0 (-0.14, 0.14)
4.3 (2.5, 8.2) 5 5 1.0 0.57-1.0 5 1.0 0.57-1.0 0 (-0.45, 0.45)
Lettuce LB/25g/24 hr
S. Newport
DD1261
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.40 (0.22, 0.64) 20 4 0.20 0.08-0.42 4 0.2 0.08-0.42 0 (-0.14, 0.14)
4.3 (2.5, 8.2) 5 5 1.0 0.57-1.0 5 1.0 0.57-1.0 0 (-0.45, 0.45)
Lettuce LB/25g/24 hr +
regrowth
S. Newport
DD1261
Control 5 0 0 0-0.43 0 0 0-0.43 0 (-0.45, 0.45)
0.40 (0.22, 0.64) 20 3 0.15 0.05-0.36 4 0.2 0.08-0.42 -0.05 (-0.29, 0.19)
4.3 (2.5, 8.2) 5 5 1.0 0.57-1.0 5 1.0 0.57-1.0 0 (-0.45, 0.45)
Dry pet food BPW/375g/24+ regrowth
S. Tennessee
DD13062
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.29 (0.13, 0.53) 20 5 0.25 (0.11, 0.47) 5 0.25 (0.11, 0.47) 0 (-0.14, 0.14)
Dry pet food LB/375g/24 +regrowth
S. Tennessee
DD13062
Control 5 0 0 (0, 0.43) 0 0 (0, 0.43) 0 (-0.45, 0.45)
0.29 (0.13, 0.53) 20 5 0.25 (0.11, 0.47) 5 0.25 (0.11, 0.47) 0 (-0.14, 0.14)
Collaborative Study Approved Protocol
Expert Review Panel Use Only