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significantly reducing the potential for contamination with one or more molecules of amplified PCR
product.
This automated BAX® System method uses fluorescent detection to analyze PCR product. One PCR
primer for each target (one
Salmonella-
specific target and an internal control) contains a fluorescent dye
(two different dyes, one for each target) as a constituent of the primer as well as a quencher (the uni-
molecular combination of a primer, fluorescent dye and quencher constitute a Scorpion™ Probe). When
incorporated into a PCR product, the dye and quencher are spatially separated, which causes an
increase in emission signal. The BAX® System measures the magnitude and characteristics of fluorescent
signal change. An analysis by the BAX® System software algorithm then evaluates that data to determine
a positive or negative result which is displayed as described below.
BAX
®
System Real-Time PCR Assay for
Salmonella
Estimated Official Start Date
It is estimated that January 14
th
, 2013 will be the first week of testing. Samples will be shipped to be
received on January 11
th
, 2013, the Friday before testing and stored at
2-8
o
C until analysis on Monday.
Study Coordination
The Study Directors are responsible for providing the test portions, clarifying procedures, collating the
results, and submitting a final report to AOAC INTERNATIONAL. Should any questions arise before or
during the course of the study, please direct them immediately to the attention of one of the co-Study
Directors.
A conference call will be schedule prior to study initiation to discuss any questions that you
may have about the study design, methods and/or workflow outlined in this protocol. The co-Study
Directors for this collaborative study are:
Morgan Wallace, Ph.D.
Patrick Bird
DuPont Qualicon
Q Laboratories, Inc.
ESL Bldg. 400
1400 Harrison Avenue
PO Box 80400
Cincinnati, Ohio 45214
Route 141 & Henry Clay Rd.
Phone : 513-471-1300
Wilmington, DE 19880
Fax : 513-471-5600
Phone: 302-695-5473
Email:
pbird@qlaboratories.comFax : 302-695-5877
Collaborative Study Design
The study will include two matrices to be tested for
Salmonella
species:
orange juice (25 g sample size)
and hot dogs (325 g sample size)
Collaborative Study Approved Protocol
Expert Review Panel Use Only