AOAC RI ERP Final Action Recommendations

AOAC INTERNATIONAL

12. STANDARD REFERENCE CULTURES

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DuPont Qualicon Culture Collection (DD) - proprietary

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American Type Culture Collection

(ATCC) -

American Type Culture Collection (ATCC) -

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www.atcc.org ,

American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA

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20108, USA.

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158

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13. MEDIA AND KIT COMPONENTS

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Items 1-4 are available as part of the BAX® System Real-Time PCR assay for

Salmonella

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1. BAX® System PCR tubes (12 strips of 8 tubes containing one PCR tablet per tube)

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2. BAX® System lysis buffer (two bottles of 12 mL each);

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3. BAX® System protease (1 vial of 400

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4. Flat optical caps (12 strips of 8 caps)

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5. Lactose broth – Dissolve 3 g of beef extract, 5 g of peptone and 5 g of lactose into 1 L of

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distilled water. Dispense into containers and autoclave 15 minutes at 121ºC. Final pH is 6.9+/-0.2

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6. BAX® System MP media – DuPont Qualicon catalog D12404925 (bulk powder), D12745725

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(StatMedia)

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7. Modified Tryptic Soy Broth containing glucose and 2 mg/L novobiocin (TSB + n), Difco

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catalog number 211825 or equivalent, BD, Franklin Lakes, NJ, 07417, USA

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8. Buffered Peptone Water (BPW) – Oxoid CM 0509 or equivalent, Oxoid, Hampshire, United

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Kingdom, RG24 8PW

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9. Brain Heart Infusion (BHI) broth – Oxoid CM 1032 or equivalent.

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10. Rappaport-Vassiliadis Soya Peptone (RVS), Selenite Cystine (SC), Tetrathionate-Hajna (TT-

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Hajna) and Tetrathionate (TT) broths and Xylose Lysine Desoxycholate (XLD), Hektoen Enteric

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Dupont BAX Salmonella PTM Report

PTM Certification No. 081201 (08/07/2012)

For Expert Review Panel Use Only

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