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AOAC INTERNATIONAL
Sample Enrichment
207
Samples were prepared for testing with either the BAX® System method or the appropriate
208
reference culture method as summarized in Table 1. Meat, poultry, and pet food samples
209
were enriched in Buffered Peptone Water according to the USDA-FSIS and/or Compendium
210
methods for reference method testing, other sample types were enriched in LB as per the
211
FDA-BAM, while BAX® System samples were enriched in either BAX® System MP media,
212
TSB + novobiocin, and/or tested directly from the reference method enrichments. All
213
enrichments, without respect to presumptive result, subject to culture confirmation and
214
isolates were biochemically and serologically confirmed by the appropriate culture
215
methodology where suspect colonies were present, with at least one confirmed colony
216
obtained from an enrichment being counted as a positive sample.
217
218
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Table 1. Matrices Tested, Reference Comparison
Method, and Strains Used
Matrix
Inoculating Strain
Reference
Method(s)
Ground Beef
DD13017
S
. Heidelberg
MLG, HC
Chicken Rinse
Naturally Occurring
MLG
Bagged Lettuce DD1261
S
. Newport
BAM
Cream Cheese
DD586
S
. Typhimurium
BAM
Dry Pet Food
DD13062
S
. Tennessee
HC
Environmental
sponges
DD12960
S
. Senftenberg BAM
Environmental
sponges
DD12960
S
. Senftenberg MLG, HC
220
MLG = USDA-FSIS Microbiological Laboratory Guidebook [4]
221
HC = Health Canada Compendium of Analytical Methods [3]
222
BAM = U.S. FDA Bacteriological Analytical Manual [2]
223
224
Ground Beef
– The BAX® System method was compared to the USDA-FSIS (USDA) and Health
225
Canada (HC) reference methods (which share the same primary enrichment media). A master
226
Dupont BAX Salmonella PTM Report
PTM Certification No. 081201 (08/07/2012)
For Expert Review Panel Use Only
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