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(HE), Brilliant Green Sulfa (BGS) and Bismuth Sulfite (BS) agars and other appropriate
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confirmatory media for reference method and alternate culture method confirmation.
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11. Lysis reagent – Pipette 150
µ
L of protease into one 12-mL bottle of lysis buffer. Label bottle
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with the date prepared. Reagent will remain stable for up to two weeks if stored at 2-8ºC.
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14. SAFETY PRECAUTIONS
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Kits
— Reagents used in the BAX® System
should pose no hazards when used as directed.
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Dispose of lysate, PCR mixture and other waste according to your site practices.
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Cycler/detector
— Only qualified laboratory personnel should operate the cycler/detector. Do not
187
attempt to repair the instrument. Live power may still be available inside the unit even when a
188
fuse has blown or been removed. Refer to the BAX® System User Guide for maintenance
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procedures when cleaning the unit or changing a fuse. The heating block can become hot enough
190
during normal operation to cause burns or cause liquids to boil. Wear safety glasses or other eye
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protection at all times during operation.
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Enrichment Broths
— All enrichment broths may contain varying pathogens whether they contain
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Salmonella
or not and thus should be sterilized and disposed of using proper procedures
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following any culture-based confirmatory steps.
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15. GENERAL PREPARATION / SAMPLE PREPARATION AND
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RECOVERY
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Note on Selection of Reference Methods
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Since many United States end users require some degree of validation of a rapid method compared to
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either the FDA-BAM or USDA-MLG as appropriate, and Canadian end users require the presentation
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of similar data comparing the appropriate Compendium method to a proposed alternative method,
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these studies were conducted using each of these three methods for at least one matrix comparison.
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Dupont BAX Salmonella PTM Report
PTM Certification No. 081201 (08/07/2012)
For Expert Review Panel Use Only
Do Not Distribute