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AOAC INTERNATIONAL
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10. ADDITIONAL REAGENTS
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Lysis reagent
– Using test kit reagents, pipette 150
µ
L of protease into one 12-mL bottle of lysis
128
buffer. Label bottle with the date prepared. Reagent will remain stable for up to two weeks if
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stored at 2-8ºC.
130
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11. APPARATUS
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Incubators
– For maintaining media at 35
±
1ºC and 39-42ºC.
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Stomacher
– Seward model 400 or equivalent for mixing the sponge sample with enrichment
135
media.
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BAX
®
System apparatus
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BAX® System Q7 cycler/detector with computer workstation
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BAX® System application software
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Cluster tubes with caps and racks for lysis
140
Capping/de-capping tools – for removing and sealing cluster tube caps and PCR
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tube caps without jarring the contents
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Thermal block(s) with inserts – for maintaining lysis tubes at 37±2ºC, 95±3ºC, and
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4±3°C.
144
Pipettes – for transferring reagents; two adjustable mechanical pipettes covering 20-
145
200
µ
L and 5-50
µ
L; one repeating pipette; and one multi-channel pipette covering 8
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channels and 5-50
µ
L. Pipettes should be calibrated to deliver required volumes
147
within 10%.
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Pipette tips with barriers: 0.5-250
µ
L, 0.5-100
µ
L extended barrier; 5 mL repeater
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pipette tips
150
Dupont BAX Salmonella PTM Report
PTM Certification No. 081201 (08/07/2012)
For Expert Review Panel Use Only
Do Not Distribute