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Revised Mar 2014
6
(a)
3M Molecular Detection System
– Available from 3M Food Safety (St. Paul, MN
1
55144-1000, USA).
2
(b)
3M Molecular Detection Assay Salmonella reagent tubes-
12 strips of 8 tubes.
3
Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
4
(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
5
(d)
Extra caps
– 12 strips of 8 caps
6
(e)
Negative control (NC)
- One vial (2 mL).
7
(f)
Reagent Control –
8 reagent tubes
8
(g)
Quick Start Guide
9
(h)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
10
(St. Paul, MN 55144-1000, USA).
11
(i)
3M
™
Molecular Detection Chill Block Tray and Chill block insert
- Available from
12
3M
™
Food Safety (St. Paul, MN 55144-1000, USA).
13
(j)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
14
(St. Paul, MN 55144-1000, USA).
15
(k)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M
16
Food Safety (St. Paul, MN 55144-1000, USA).
17
(l)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M
18
Food Safety (St. Paul, MN 55144-1000, USA).
19
(m)
Empty lysis tube rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000,
20
USA).
21
(n)
Empty reagent tube rack
- Available from 3M Food Safety (St. Paul, MN 55144-
22
1000, USA).
23
(o)
3M
™
Buffered Peptone Water [ISO] (3M BPW ISO)
– Available from 3M Food
24
Safety (St. Paul, MN 55144-1000, USA). (Formulation equivalent to ISO 6579:2002
25
Annex B) [4]
26
(p)
Rappaport Vassiliadis [R10]
– Availabile from 3M Food Safety (St. Paul, MN
27
55144-1000, USA).
28
(q)
Disposable pipette
– capable of 20 µL
29
(r)
Multi
-channel (8-channel) pipette - capable of 20 µL
30
(s)
Sterile filter tip pipette tips
- capable of 20 µL
31
(t)
Filter Stomacher® bags –
Seward or equivalent
.
32
(u)
Stomacher
®– Seward or equivalent.
33
(v)
Thermometer –
calibrated range to include 100 ± 1
o
C
34
(w)
Dry double block heater unit or water bath –
capable of maintaining 100 ± 1
o
C
35
(x)
Incubators.
– Capable of maintaining 37 ± 1°C, 41.5 ±1°C
36
(y)
Freezer –
capable of maintaining -10 to -20
o
C, for storing the 3M Molecular
37
Detection Chill Block Tray
38
(z)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular Detection
39
Assay
40
(aa)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
41
42
C. Safety Precautions
43
The 3M™ Molecular Detection Instrument is intended for use with samples that have
44
undergone heat treatment during the assay lysis step, which is designed to destroy organisms
45
present in the sample. Samples that have not been properly heat treated during the assay
46