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Revised Mar 2014
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NOTE:
The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C before
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inserting the 3M Molecular Detection Speed Loader Tray with reaction tubes. This heating step takes
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approximately 20 minutes and is indicated by an ORANGE light on the instrument’s status bar. When the
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instrument is ready to start a run, the status bar will turn GREEN.
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F.
Analysis
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Lysis
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1.
Allow the lysis solution (LS) tubes to warm up to room temperature by setting the
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rack on the laboratory bench for 2 hours
(a)
.
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2.
Remove the enrichment broth from the incubator and gently agitate the contents.
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3.
One LS tube is required for each sample and the Negative Control (NC) sample.
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3.1
LS tube strips can be cut to desired LS tube number. Select the number of
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individual LS tubes or 8-tube strips needed. Place the LS tubes in an empty rack.
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3.2
To avoid cross-contamination, decap one LS tubes strip at a time and use a new
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pipette tip for each transfer step.
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4.
Transfer enriched sample to LS tubes as described below:
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Transfer each enriched sample into individual LS tube
first
. Transfer the NC
last
.
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4.1
Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one LS tube
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strip - one strip at a time. Set the tool with cap attached aside on a clean surface.
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4.2
Transfer 20 µL of sample into a LS tube.
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4.3
Repeat step 4.2 until each individual sample has been added to a corresponding LS
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tube in the strip
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4.4
Use the 3M Molecular Detection Cap/Decap Tool-Lysis to re-cap the LS tube strip.
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Use the rounded side of the tool to apply pressure in a back and forth motion
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ensuring that the cap is tightly applied.
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4.5
Repeat steps 4.1 to 4.4 as needed, for the number of samples to be tested
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4.6
When all samples have been transferred, then transfer
20 µL of NC into a LS
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tube
. Use the 3M Molecular Detection Cap/Decap Tool-Lysis tool to re-cap the LS
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tube.
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4.7
Cover the rack of LS tubes with the rack lid and firmly invert 3-5 times to mix.
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Suspension has to flow freely inside the tube.
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5.
Verify that the temperature of the 3M Molecular Detection Heat Block Insert is at 100
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±1°C. Place the rack of LS tubes in the 3M Molecular Detection Heat Block Insert and
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heat for 15 ±1 minutes
(b)
. Samples that have not been properly heat treated during the
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