319 / 1195
Revised Mar 2014
9
Sample
Matrix
Sample
Size
Enrichment
Broth
Volume
(mL)
Enrichment
Temperature
(C)
Enrichment
Time (hr)
Secondary
Enrichment
Medium
(mL)
Secondary
Enrichment
Temperature
©
Secondary
Enrichment
Time (hr)
Raw shrimp,
head on
25 g
225 mL
37
18-14
RV[R10]:
0.1 mL into
10 mL
41.5
4-24
1
2
Preparation of the 3M™ Molecular Detection Speed Loader Tray
3
1.
Wet a cloth or paper towel with a 1-5% (v:v in water) household bleach solution and
4
wipe the 3M™ Molecular Detection Speed Loader Tray.
5
2.
Rinse the 3M Molecular Detection Speed Loader Tray with water.
6
3.
Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.
7
4.
Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.
8
9
Preparation of the 3M™ Molecular DetectionChill Block Insert
10
Before using the 3M™ Molecular Detection Chill Block Insert, ensure it has been
11
stored on the 3M™ Molecular Detection Chill Block Tray in the freezer (-10 to -20°C)
12
for a minimum of 2 hours before use. When removing the 3M Molecular Detection
13
Chill Block Insert from the freezer for use, remove it and the 3M Molecular Detection
14
Chill Block Tray together. Use the 3M Molecular Detection Chill Block Insert /3M
15
Molecular Detection Chill Block Tray within 20 minutes.
16
Preparation of the 3M™ Molecular Detection Heat Block Insert
17
Place the 3M™ Molecular Detection Heat Block Insert in a dry double block heater
18
unit. Turn on the dry block heater unit and set the temperature to allow the 3M
19
Molecular Detection Heat Block Insert to reach and maintain a temperature of 100
20
±1°C.
21
NOTE:
Depending on the heater unit, allow approximately 30-50 minutes for the 3M
22
Molecular Detection Heat Block Insert to reach temperature. Using a calibrated
23
thermometer, verify that the 3M Molecular Detection Heat Block Insert is at 100 ±1°C.
24
Preparation of the 3M Molecular Detection Instrument
25
1.
Launch the 3M™ Molecular Detection Software and log in.
26
2.
Turn on the 3M Molecular Detection Instrument.
27
3.
Create or edit a run with data for each sample. Refer to the 3M Molecular Detection
28
System User Manual for details.
29
30