317 / 1195
Revised Mar 2014
7
lysis step may be considered a potential biohazard and should NOT be inserted into the 3M
1
Molecular Detection Instrument.
2
3
After use, the enrichment medium and the 3M Molecular Detection Assay
Salmonella
tubes
4
can potentially contain pathogenic materials. When testing is complete, follow current
5
industry standards for the disposal of contaminated waste. Consult the Material Safety Data
6
Sheet for additional information and local regulations for disposal.
7
8
D.
MethodPreparation and Precautions
9
The 3M Molecular Detection Assay
Salmonella
is intended for use in a laboratory
10
environment by professionals trained in laboratory techniques. The user should read,
11
understand and follow all safety information in the instructions for the 3M Molecular
12
Detection System and the 3M Molecular Detection Assay
Salmonella
and retain the safety
13
instructions for future reference. Follow all instructions carefully. Failure to do so may lead
14
to inaccurate results.
15
16
Store the 3M Molecular Detection Assay
Salmonella
at 2-8°C. Do not freeze. Keep kit away
17
from light during storage. After opening the kit, check that the foil pouch is undamaged. If
18
the pouch is damaged, do not use. After opening, unused reagent tubes should always be
19
stored in the re-sealable pouch with the desiccant inside to maintain stability of the
20
lyophilized reagents. Store resealed pouches at 2-8°C for no longer than 1 month. Do not
21
use 3M Molecular Detection Assay
Salmonella
past the expiration date. Expiration date and
22
lot number are noted on the outside label of the box.
23
24
Use proper aseptic technique. Use proper precautions for biosafety level 2 microorganisms.
25
Periodically laboratory benches and equipment (pipettes, cap/decap tools, etc…) should be
26
decontaminated with a 1-5% (v:v in water) household bleach solution or DNA removal
27
solution.
28
29
30
E.
Sample Preparation
31
Table 1 presents guidance for the enrichment of food and feed samples at a 1:10 dilution. It
32
is the user’s responsibility to validate alternate sampling protocols or dilution ratios to
33
ensure this test method meets the user’s criteria.
34
1.
Pre-warm 3M BPW ISO enrichment medium to 37 ±1°C or 41.5 ±1°C.
35
2.
Aseptically combine the enrichment medium and sample. Homogenize thoroughly
36
for 2 minutes Incubate at 37 ±1°C or 41.5 ±1°C.
37
For all meat and highly particulate samples, the use of filter bags is recommended.
38
In an AOAC Research Institute Performance Tested Method study, the 3M Molecular Detection
39
Assay
Salmonella
was found to be an effective method for the detection of Salmonella in the
40
following matrices:
41