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3M Molecular Detection Assay Salmonella Collaborative Study
July 2012
OMA-2012-July-XXX
4.6
Most Probable Number (MPN) Analysis.
To determine the level of
Salmonella
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spp
.
in the inoculated food test portions on the day of initiation of analysis, a 5-
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tube MPN determination is performed by the coordinating laboratory following
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the appropriate reference method. From each of the inoculated food batches,
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prepare 5 test portions of 100 g, 5 test portions of 25 g and 5 test portions of 10 g.
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To each test portion, add the appropriate reference method enrichment media to
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make a 1:10 dilution. Follow the reference method through to confirmations. Use
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the number of positives from the high, medium and low levels to calculate the
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MPN for each inoculated level of each matrix. Access the MPN calculator
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( http://www.lcfltd.com/customer/LCFMPNCalculator.exe )to determine the MPN
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values and 95% confidence intervals. Data should be entered from high level to
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low. Report MPN as MPN/test portion.
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5.0
3M Molecular Detection Assay
Salmonella
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5.1
Applicability
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For the detection of
Salmonella
species in food and feed matrices: raw ground
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beef, cooked breaded chicken, raw shrimp, bagged spinach, wet pet food, and
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pasteurized liquid whole egg.
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Caution
:
The 3M Molecular Detection Assay
Salmonella
is intended for use
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in a laboratory environment by professionals trained in laboratory techniques.
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The user should read, understand and follow all safety information in the
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instructions for the 3M Molecular Detection System and the 3M Molecular
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Detection Assay
Salmonella
and retain the safety instructions for future
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reference. Follow all instructions carefully. Failure to do so may lead to
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inaccurate results.
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The 3M Molecular Detection Instrument is intended for use with samples that
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have undergone heat treatment during the assay lysis step, which is designed
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to destroy organisms present in the sample. Samples that have not been
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properly heat treated during the assay lysis step may be considered a potential
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biohazard and should NOT be inserted into the 3M Molecular Detection
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Instrument.
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Store the 3M Molecular Detection Assay
Salmonella
at 2-8°C. Do not freeze.
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Keep kit away from light during storage. After opening the kit, check that the
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foil pouch is undamaged. If the pouch is damaged, do not use. After opening,
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unused reagent tubes should always be stored in the re
‑
sealable pouch with
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the desiccant inside to maintain stability of the lyophilized reagents. Store
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resealed pouches at 2-8°C for no longer than 1 month. Do not use 3M
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Molecular Detection Assay
Salmonella
past the expiration date. Expiration
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date and lot number are noted on the outside label of the box.
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DRAFT DOCUMENT
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