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3M Molecular Detection Assay Salmonella Collaborative Study
July 2012
OMA-2012-July-XXX
After use, the enrichment medium and the 3M Molecular Detection Assay
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Salmonella
tubes can potentially contain pathogenic materials. When testing
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is complete, follow current industry standards for the disposal of
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contaminated waste. Consult the Material Safety Data Sheet for additional
4
information and local regulations for disposal.
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5.2
Test Kit Reagents
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5.2.1
The 3M Molecular Detection Assay
Salmonella
- 96 tests
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5.2.1.1
Lysis Solution (LS) tubes
. – 96 (12 strips of 8 tubes)
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5.2.1.2
Salmonella Reagent tubes
. – 96 (12 strips of 8 tubes)
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5.2.1.3
Extra caps.
– 96 (12 strips of 8 caps)
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5.2.1.4 Negative Control (NC). – 1 tube
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5.2.1.5 Reagent Control (RC). – 8 tubes
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5.2.1.6
Quick Start Guide
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5.3
Additional Supplies and Reagents
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5.3.1
3M Molecular Detection Instrument, power supply, cord, USB cable and
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software
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5.3.2
3M Molecular Detection Speed Loader Tray
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5.3.3
3M Molecular Detection Chill Block Tray and Chill block insert
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5.3.4
3M Molecular Detection Heat Block Insert
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5.3.5
3M Molecular Detection Cap/Decap Tool [Reagent]
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5.3.6
3M Molecular Detection Cap/Decap Tool [Lysis]
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5.3.7
Empty lysis tube rack
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5.3.8
Empty reagent tube rack
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5.3.9
3M Buffered Peptone Water [ISO] (3M BPW ISO)
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5.4
Selective agar
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5.4.1
Hektoen Agar (HE)
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5.4.2
XLD Agar
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5.4.3
XLT4 Agar
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5.4.4
Bismuth Sulfite (BS)
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5.4.5
Brilliant Green Sulfa (BGS)
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5.5
Apparatus
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5.5.1
Pipettes
. – capable of 20µL
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5.5.2
Multi-channel (8-channel) pipette
. – capable of 20µL
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5.5.3
Sterile filter-tip pipette tips.
– capable of 20µL
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5.5.4
Stomacher®
. – Seward or equivalent
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5.5.5
Filter Stomacher® bags
. – Seward or equivalent
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5.5.6
Thermometer
. – calibrated range to include 100±1°C range
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5.5.7
Dry double block heater unit.
– capable of maintaining 100 + 1°C;
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OR
a water bath -
capable of maintaining 100 + 1°C
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5.5.8
Incubators
. – capable of maintaining 37 ± 1°C
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5.5.9
Freezer
. – capable of maintaining -10 to -20°C, for storing the 3M
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Molecular Detection Chill Block Tray
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DRAFT DOCUMENT
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